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KMID : 0358319930340040748
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Korean Journal of Urology
1993 Volume.34 No. 4 p.748 ~ p.763
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Experimental Study on the Effect of Suramin on Penile Squamous Carcinoma Cell Line(CUPE-1)
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Abstract
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Experimental study was done to investigate the effect of suramin on the in vitro and in vivo proliferation and metastasis of penile squamous carcinoma cell line (CUPE-1) , morphological changes of CUPE-1 cells induced by suramin and mechanism of
action
of suramin.
Suramin inhibited in vitro proliferation of CUPE-1 significantly with IC50 of 100μg/ml media. In vitro antiproliferative effect of suramin on CUPE-1 was reversible after stopping administration of the drug. Weekly intraperitoneal of 200mg/kg of
suramin
to nude mouse inhibited the proliferation and metastasis of intraperitoneally implanted CUPE-1 cells significantly. but did not show significant effect on the proliferation of subcutaneously implanted CUPE-1 cells. Seramin induced senile changes
on
ultrastructure of CUPE-1 cells. Suramin of 300μg/ml inhibited the proliferation-stimulating effect of EGF significantly, whereas, suramin of 100μg/ml did not inhibit the effect of EGF significantly. Suramin did not show significant cytotoxicity
on
3H-thymidine release assay.
These results suggest that suramin is a promising drug for the treatment of advanced penile squamous cell carcinoma and blood level of suramin in clinical trial should be continuously maintained in about 300μg/ml, and that the main machanism of
suramin
against CUPE-1 is cytostatic, by antagonizing the action of EGF and inducing growth arrest an senile change.
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